Enantiomeric separation of basic compounds using heptakis(2,3-di-O-methyl-6-O-sulfo)-β-cyclodextrin in combination with potassium camphorsulfonate in nonaqueous capillary electrophoresis: Optimization by means of an experimental design
✍ Scribed by Anne-Catherine Servais; Marianne Fillet; Patrice Chiap; Walthère Dewé; Philippe Hubert; Jacques Crommen
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 242 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The enantiomeric separation of a series of basic pharmaceuticals (β‐blockers, local anesthetics, sympathomimetics) has been investigated in nonaqueous capillary electrophoresis (NACE) systems using heptakis(2,3‐di‐O‐methyl‐6‐O‐sulfo)‐β‐cyclodextrin (HDMS‐β‐CD) in combination with potassium camphorsulfonate (camphorSO~3~^−^). For this purpose, a face‐centered central composite design with 11 experimental points was applied. The effect of the concentrations of HDMS‐β‐CD and camphorSO~3~^−^ on enantioresolution was statistically evaluated and depended largely on the considered analyte. The presence of camphorSO~3~^−^ was found to be particularly useful for the enantioseparation of compounds with high affinity for the anionic CD. CamphorSO~3~^−^ seems to act as a competitor, reducing the affinity for the CD, probably by ion‐pair formation with these analytes. For compounds with lower affinity for HDMS‐β‐CD, the combination of camphorSO~3~^−^ and the CD appeared to have a favorable effect on enantioresolution only if the optimal CD concentration could be reached. On the other hand, for compounds characterized by a very low affinity for the anionic CD, the association of camphorSO~3~^−^ and HDMS‐β‐CD is always unfavorable. Finally, experimental conditions were selected by means of the multivariate approach in order to obtain the highest resolution (R~s~) value for each studied compound.