Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamines in abusers' hair and urine

✍ Scribed by Osama Al-Dirbashi; Mitsuhiro Wada; Naotaka Kuroda; Shigeko Inuduka; Kenichiro Nakashima

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 78 KB
• Volume: 13
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199912)13:8<543::aid-bmc925>3.0.co;2-7

✦ Synopsis

Enantiomer-specific high-performance liquid chromatography with fluorescence detection using 4-(4,5-diphenyl-1Himidazol-2-yl)-benzoyl chloride as a fluorescence labeling reagent was applied to determine methamphetamine and its metabolites in abusers' hair and urine. Hair samples were segmentally analyzed based on 1 cm long segments. In four hair samples, only the S()enantiomers of methamphetamine and its N-demethylated metabolite, S()-amphetamine were detected. Satisfactory correlation (r = 0.901) between the results of high-performance liquid chromatography-fluorescence and those of gas chromatography-nitrogen phosphorous detection was obtained (n = 19). In an abuser's urine sample, the S()-and R(À)-enantiomers of methamphetamine, amphetamine and para-hydroxymethamphetamine were detected. The degree of N-demethylation of S()-methamphetamine into the corresponding metabolite of amphetamine was significantly higher than that of the R(À)-enantiomer.