Enantiomer separation on monolithic silica HPLC columns using chemically bonded methylated and methylated/acetylated 6-O-tert-butyldimethyl- silylated β-cyclodextrin
✍ Scribed by Mathias Bayer; Clarissa Hänsel; Armin Mosandl
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 906 KB
- Volume
- 29
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Enantiomer separation on monolithic silica HPLC columns using chemically bonded methylated and methylated/acetylated 6-O-tert-butyldimethylsilylated b-cyclodextrin* A new 2,3-methylated 3*-monoacetylated 6-O-tert-butyldimethylsilylated b-CD derivative was synthesized and chemically bonded onto aminopropyl derivatized monolithic silica HPLC columns. In this CD derivative, only one of seven methyl groups in 3-position was substituted by an acetyl group. Its applicability as a chiral stationary phase for HPLC was tested and compared with exclusively 2,3-methylated 6-O-tertbutyldimethylsilylated b-CD immobilized onto aminopropyl-modified monoliths. Thirty-two chiral compounds from different chemical classes and different functionalities were tested under RP conditions. Fourteen compounds were resolved into their enantiomers by methylated 6-O-tert-butyldimethylsilylated b-CD. By use of methylated/acetylated 6-O-tert-butyldimethylsilylated b-CD as the chiral stationary phase 7 analytes were successfully stereodifferentiated.