Enantiomer separation of 7-des-methyl-ormeloxifene using sulfated β-cyclodextrin in countercurrent chromatography

✍ Scribed by Jens Breinholt; Søren Vig Lehmann; Annemarie Reinhardt Varming

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 86 KB
• Volume: 11
• Category: Article
• ISSN: 0899-0042
• DOI: 10.1002/(sici)1520-636x(1999)11:10<768::aid-chir5>3.0.co;2-e

✦ Synopsis

The enantiomers of 7-des-methyl-ormeloxifene were separated by countercurrent chromatography (CCC) using sulfated ␤-cyclodextrin as chiral selector, representing the first reported successful application of a cyclodextrin derivative in CCCbased resolutions. The choice of chiral selector relies on extreme separation factors observed in chiral capillary electrophoresis, and suitable CCC conditions were developed employing an analytical toroidal coil countercurrent chromatograph. Preparative separation of the enantiomers was performed using a conventional, preparative CCCinstrument.