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Empfindlicher Nachweis von p-Nitrophenol in Blut und Urin mit HPLC nach E 605-Intoxikation

✍ Scribed by Michalke, P.


Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
374 KB
Volume
88
Category
Article
ISSN
1437-1596

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✦ Synopsis


p-Nitrophenol, the main metabolite of parathion metabolism can be quantified by high performance liquid chromatography (HPLC) following very simple preparation of small quantities of blood and urine.

Determination is carried out by UV detection at 315nm following separation on a RP 18 column. Detection limits are 5-10ng/ml serum and 50-100 ng/ml urine.

Levels of p-nitrophenol in blood and urine after survival from parathion intoxication are demonstrated.