𝔖 Bobbio Scriptorium
✦   LIBER   ✦

EMF acts on rat bone marrow mesenchymal stem cells to promote differentiation to osteoblasts and to inhibit differentiation to adipocytes

✍ Scribed by Yong Yang; Chaoxiong Tao; Dongming Zhao; Feng Li; Wenchun Zhao; Hua Wu

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
263 KB
Volume
31
Category
Article
ISSN
0197-8462

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The use of electromagnetic fields (EMFs) to treat nonunion fractures developed from observations in the mid‐1900s. Whether EMF directly regulates the bone marrow mesenchymal stem cells (MSCs), differentiating into osteoblasts or adipocytes, remains unknown. In the present study, we investigated the roles of sinusoidal EMF of 15 Hz, 1 mT in differentiation along these separate lineages using rat bone marrow MSCs. Our results showed that EMF promoted osteogenic differentiation of the stem cells and concurrently inhibited adipocyte formation. EMF increased alkaline phosphatase (ALP) activity and mineralized nodule formation, and stimulated osteoblast‐specific mRNA expression of RUNX2, ALP, BMP2, DLX5, and BSP. In contrast, EMF decreased adipogenesis and inhibited adipocyte‐specific mRNA expression of adipsin, AP‐2, and PPARγ2, and also inhibited protein expression of PPARγ2. These observations suggest that commitment of MSCs into osteogenic or adipogenic lineages is influenced by EMF. Bioelectromagnetics 31:277–285, 2010. © 2009 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Human peripheral blood derived mesenchym
Human peripheral blood derived mesenchymal stem cells demonstrate similar characteristics and chondrogenic differentiation potential to bone marrow derived mesenchymal stem cells
✍ Pan-Pan Chong; Lakshmi Selvaratnam; Azlina A. Abbas; Tunku Kamarul 📂 Article 📅 2011 🏛 Elsevier Science 🌐 English ⚖ 928 KB

## Abstract The use of mesenchymal stem cells (MSCs) for cartilage repair has generated much interest owing to their multipotentiality. However, their significant presence in peripheral blood (PB) has been a matter of much debate. The objectives of this study are to isolate and characterize MSCs de

Osteoblastic differentiation of human an
Osteoblastic differentiation of human and equine adult bone marrow-derived mesenchymal stem cells when BMP-2 or BMP-7 homodimer genetic modification is compared to BMP-2/7 heterodimer genetic modification in the presence and absence of dexamethasone
✍ Ryan S. Carpenter; Laurie R. Goodrich; David D. Frisbie; John D. Kisiday; Beth C 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 268 KB 👁 1 views

## Abstract Bone marrow‐derived mesenchymal stem cells (BMDMSCs) have been targeted for use in enhancement of bone healing; and their osteogenic potential may be further augmented by genes encoding bone morphogenetic proteins (BMP's). The purpose of this study was to compare the effect of genetic m