EMCCD Camera Technology Advances : New EMCCD Camera Offers 16-Bit Quantitative Stability for Ratiometric Imaging
✍ Scribed by Deepak Sharma
- Book ID
- 102866563
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2006
- Weight
- 715 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1439-4243
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✦ Synopsis
Several years ago, Photometrics' introduction of the first electron-multiplying CCD (EMCCD) cameras designed for microscopy enabled life science researchers to detect ultra-low-light signals via on-chip multiplication gain. Since the advent of EMCCDs, camera manufacturers have focused their R&D efforts on areas that offer the most room for improvement of such devices, including bias and EM gain stability as well as sensor aging. Here we highlight the development of a new imaging platform that provides a greater capacity to control EMCCDs, thus allowing biological researchers to quantitatively measure low-light signals with extremely high fidelity. Advantages in high-speed measurement of a calcium ratiometric time-course application are demonstrated.