Embryotoxic effects of heterologous antisera against rat reichert's membrane

✍ Scribed by Leung, Christopher C. K.

• Book ID: 102892453
• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 807 KB
• Volume: 200
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402000210

✦ Synopsis

Abstract

Parietal yolk‐sacs of rat embryos at the fifeenth day of gestation were obtained by microdissection. A Reichert's membrane (RM) preparation was isolated by treating the parietal yolk‐sacs with the chelating agent tetrasodium salt of ethylenediaminetetraacetic acid (EDTA) combined with mechanical shaking. Less than 1% of the membrane preparation was DNA and phosphorus contaminants. The membrane purity was also evaluated by electron microscopic examination. Rabbit Ig G directed against the RM preparation when injected ip into ninth day pregnant rats produced malformations, fetal growth retardation and resorption. Fluorescent‐labeled antibody localization studies demonstrated that the teratogenic antibodies localized in RM. It is postulated that RM antibodies induce teratogenesis by interfering with the function of RM.