Embryonic Stem Cell Protocols: Volume 1: Isolation and Characterization

✍ Scribed by Yunhan Hong, Manfred Schartl (auth.), Kursad Turksen (eds.)

• Publisher: Humana Press
• Year: 2006
• Tongue: English
• Leaves: 534
• Series: Methods in Molecular Biology 329
• Edition: 2
• Category: Library

✦ Synopsis

Drawing on the dramatically increasing research on embryonic stem (ES) cell biology and differentiation, Kursad Turksen has completely updated and expanded his highly acclaimed first edition of Embryonic Stem Cells: Methods and Protocols into two volumes. Focusing on ES cells recently isolated from different nonhuman species, volume one of Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. A companion second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, the two volumes of Embryonic Stem Cells illuminate for both novices and experts not only our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications, but also provide detailed accounts of the tools required for successful work in the area.

✦ Table of Contents

Front Matter....Pages i-xxi
Front Matter....Pages 1-1
Front Matter....Pages 3-16
Front Matter....Pages 17-34
Front Matter....Pages 35-44
Front Matter....Pages 45-58
Front Matter....Pages 59-79
Front Matter....Pages 81-89
Front Matter....Pages 91-98
Back Matter....Pages 99-99
....Pages 101-112

✦ Subjects

Cell Biology