Embryonic Stem Cell Protocols, Vol. II: Differentiation Models, 2nd Edition, v330

✍ Scribed by Kursad Turksen

• Publisher: Humana Press
• Year: 2006
• Tongue: English
• Leaves: 480
• Series: Methods in Molecular Biology volume 330
• Edition: 2
• Category: Library

✦ Synopsis

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

✦ Table of Contents

1 Neural Stem Sphere Method.PDF......Page 1
2 Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In Vitro.pdf......Page 38
3 Derivation and Characterization of Neural Cells From Embryonic Stem Cells Using Nestin Enhancer.pdf......Page 56
4 Optimized Neuronal Differentiation of Murine Embryonic Stem Cells--- Role of Cell Density.pdf......Page 78
5 Generation of Inner Ear Cell Types From Embryonic Stem Cells.pdf......Page 94
6 Derivation of Epidermal Colony-Forming Progenitors From Embryonic Stem Cell Cultures.pdf......Page 116
7 Directing Epidermal Fate Selection by a Novel Co-Culture System.pdf......Page 128
8 In Vitro Generation of T Lymphocytes From Embryonic Stem Cells.pdf......Page 136
9 The Role of Hex in Hemangioblast and Hematopoietic Development.pdf......Page 146
10 Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells.pdf......Page 158
11 Analysis of Embryonic Stem Cell-Derived Osteogenic Cultures.pdf......Page 172
12 Generation of Chondrocytes From Embryonic Stem Cells.pdf......Page 184
13 Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro.pdf......Page 194
14 Generation and Characterization of Cardiomyocytes Under Serum-Free Conditions.pdf......Page 214
15 Analysis of Arrhythmic Potential of Embryonic Stem Cell-Derived Cardiomyocytes.pdf......Page 244
16 Derivation and Characterization of Alveolar Epithelial Cells From Murine Embryonic Stem Cells In Vitro.pdf......Page 256
17 Derivation and Characterization of Thyrocyte-Like Cells From Embryonic Stem Cells In Vitro.pdf......Page 272
18 Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells.pdf......Page 286
19 Formation of Gut-Like Structures In Vitro From Mouse Embryonic Stem Cells.pdf......Page 302
20 In Vitro Derivation and Expansion of Endothelial Cells From Embryonic Stem Cells.pdf......Page 310
21 Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells--- Genetic Selection and Potential Use In Vivo.pdf......Page 326
22 Integrins and Vascular Development in Differentiated Embryonic Stem Cells In Vitro.pdf......Page 354
23 TGF-β Signaling in Embryonic Stem Cell-Derived Endothelial Cells.pdf......Page 364
24THER~1.PDF......Page 376
25 In Vitro Differentiation of Embryonic Stem Cells Into the Pancreatic Lineage.pdf......Page 396
26 Derivation and Characterization of Hepatocytes From Embryonic Stem Cells In Vitro.pdf......Page 410
27 Differentiation of Embryonic Stem Cells to Retinal Cells In Vitro.pdf......Page 424
28DERI~1.PDF......Page 440
29 Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-Dimensional Collagen Matrix.pdf......Page 454