Embryogenic protoplast cultures of orange jessamine (Murraya paniculata) and their regeneration into plants floweringin vitro

✍ Scribed by Hasan Basri Jumin; Nobumasa Nito

• Book ID: 104621399
• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 518 KB
• Volume: 41
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00045092

✦ Synopsis

Embryogenic callus was induced from the hypocotyl region of seedlings germinated from immature embryos of orange jessamine (Murraya paniculata (L.) Jack) on Murashige & Tucker (1969) medium containing 50 g 1-1 sucrose, 5.0 mg 1-l benzyladenine, 2.5 mg 1-1 2,4-dichlorophenoxyacetic acid and 600 mg 1-1 malt extract. Isolated protoplasts divided to produce callus on Murashige & Tucker (1969) medium containing 50 g 1-1 sucrose, 0.01 mg 1-1 gibberellin A4+7 and 600 mg 1-l malt extract. Callus developed to plantlets via somatic embryogenesis on Murashige & Tucker (1969) medium with 50 g 1 -l lactose but no plant growth regulators. These plantlets flowered in vitro on half strength Murashige & Tucker (1969) medium containing 50 g 1-l sucrose after 2 months culture.