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Embryogenesis and plant regeneration of spinach (Spinacia oleraceaL.) from hypocotyl segments

✍ Scribed by Xing-Guo Xiao; Michel Branchard

Publisher
Springer
Year
1993
Tongue
English
Weight
478 KB
Volume
13
Category
Article
ISSN
0721-7714

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✦ Synopsis

A system for somatic embryogenesis and plant regeneration of spinach from hypocotyl segments has been established. Callus was induced on solid media supplemented with 8.5-15.0 mg.l(-1) of indole-3-acetic acid and 3.46-34.64 mg.l(-1) gibberellic acid. Callus was then subcultured on different media (solid or liquid) with or without IAA, or continuously maintained on the initiating media. Somatic embryos were obtained in subcultures on IAA-containing media as well as in long-term cultures on initiating media. The best results were achieved in liquid subcultures. About 60% of plantlets survived after transplanting in pots.

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