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Elution of glycoproteins from replicas of sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels

✍ Scribed by Dr. Boguslaw Szewczyk; Zbigniew Pilat; Krystyna Bienkowska-Szewczyk; Donald F. Summers


Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
478 KB
Volume
19
Category
Article
ISSN
0173-0835

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A highly sens,tive fluorescent staining method for detecting glycosylated proteins on SDS-polyacrylamide gel has been described. The carbohydrate groups in glycoproteins were oxidized with periodate on SDS-polyacrylamide gel to generate aldehyde groups which were subsequently labeled with a fluoresc