The effect of surfactants on protein adsorption has been The elutability of proteins from adjuvants in model vaccines recently reviewed by Arnebrant and Wahlgren (3). They composed of ovalbumin adsorbed by aluminum hydroxide adjuconcluded that surfactants may elute preadsorbed proteins vant or lysozyme adsorbed by aluminum phosphate adjuvant folby either a solubilization or replacement mechanism. Any lowing treatment with surfactant solutions was studied. Nonionic type of complex formation between protein and surfactant (Triton X-100, lauryl maltoside), zwitterionic (lauryl sulfobeis included in the solubilization mechanism. The removal of taine), anionic (sodium dodecyl sulfate), and cationic (cetylpyripreadsorbed proteins by surfactant has also been extensively dinium chloride, dodecyltrimethylammonium chloride) surfacstudied by Horbett and co-workers (4-7). They use the tants were investigated. Cetylpyridinium chloride produced the term ''elutability'' to describe the removal of a preadsorbed greatest degree of elution (60%) of ovalbumin from aluminum hydroxide adjuvant. Sodium dodecyl sulfate completely eluted ly-protein by treatment with a surfactant. In this paper we will sozyme from aluminum phosphate adjuvant. The effectiveness of use the terminology developed by Arnebrant and Wahlgren surfactants in removing preadsorbed proteins was directly related as well as by Horbett and co-workers. to their ability to denature the protein. Micellar solubilization and We are aware of only one study which focused on the electrostatic repulsion may also contribute to desorption. แญง 1998 desorption of a protein from an aluminum-containing adju-Academic Press vant (8). Phosphate anion was found to desorb ovalbumin Key Words: elutability; aluminum hydroxide adjuvant; alumifrom aluminum hydroxide adjuvant. There have been a numnum phosphate adjuvant; surfactant-induced elution; desorption ber of reports on the use of surfactants to elute proteins from of preadsorbed proteins; solubilization; micellar solubilization; other types of surfaces (4-7, 9-12). A 3% sodium dodecyl electrostatic repulsion.
sulfate (SDS) solution has been used to elute fibrinogen from a number of different polyurethane surfaces ( 13). An aging effect was noted as less fibrinogen was eluted with iep of commercial aluminum phosphate adjuvant is between 1 To whom correspondence should be addressed. 4 and 5. Thus, it is a good adsorbent for basic proteins. 48