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Elucidation of the mass fragmentation pathways of potato glycoalkaloids and aglycons using Orbitrap mass spectrometry

✍ Scribed by Michael G. Cahill; Giovanni Caprioli; Sauro Vittori; Kevin J. James

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
202 KB
Volume
45
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

The mass fragmentation of potato glycoalkaloids, α‐solanine and α‐chaconine, and the aglycons, demissidine and solasodine were studied using the Orbitrap Fourier transform (FT) mass spectrometer. Using the linear ion trap (LIT) mass spectrometry, multistage collisional‐induced dissociation (CID) experiments (MS^n^) on the [M + H]^+^ precursor ions were performed to aid the elucidation of the mass fragmentation pathways. In addition, higher energy collisional‐induced dissociation (HCD) mass spectra were generated for these toxins at a high resolution setting [100 000 FWHM (full width at half maximum)] using the Orbitrap. This hybrid mass spectrometry instrumentation was exploited to produce MS^3^ spectra by selecting MS^2^ product ions, generated using LIT MS, and fragmentation using HCD. The accurate mass data in the MS^3^ spectra aided the confirmation of proposed product ion formulae. The precursor and product ions from glycoalkaloids lost up to four sugars from different regions during MS^n^ experiments. Mass fragmentation of the six‐ring aglycons were similar, generating major product ions that resulted from cleavages at the B‐rings and E‐rings. Copyright © 2010 John Wiley & Sons, Ltd.

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