Elucidating the mechanism of chain termination switching in the picromycin/methymycin polyketide synthase
โ Scribed by Li Tang; Hong Fu; Melanie C Betlach; Robert McDaniel
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 888 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1074-5521
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## Background: Polyketides are important compounds with antibiotic and anticancer activities. several modular polyketide synthases (pkss) contain a terminal thioesterase (te) domain probably responsible for the release and concomitant cyclization of the fully processed polyketide chain. because the
E)-3-Methyl-3-pentenyl diphosphate acted as an artificial homoallylic substrate in the reaction with several allylic diphosphates catalyzed by undecaprenyl diphosphate synthase of Bacillus subtilis. The synthase reaction with the artificial substrate proceeded in the same stereochemical manner as th