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Elongational flow studies of hinged rodlike molecules

โœ Scribed by Ichiro Hayakawa; Chiaki Hayashi; Naoki Sasaki; Kunio Hikichi


Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
386 KB
Volume
61
Category
Article
ISSN
0021-8995

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โœฆ Synopsis


Poly(amino acid) in a n intermediate state of its helix-coil transition is known t o be in a hinged rodlike conformation. In this work, the responses of poly(amino acids) in the hinged rodlike conformation against a n elongational flow field were investigated by monitoring their flow-induced birefringence. Poly(L-glutamic acids) (PGA) and poly(y-benzyl-L-glutamate) (PBLG) were examined as polyelectrolyte and noncharged poly(amino acids), respectively, and the results were compared. In the plots of flow-induced birefringence, An, against strain rate, E, for hinged rodlike PBLG, there was a critical strain rate, Eo, below which An was not observed. Over Co, the birefringence pattern observed was identical with t h a t of rodlike molecules. T h e An vs. C plot for hinged rodlike PGA had characteristics of a rigid rod a t any strain rate and there was no Eo observed. T h e rotational diffusion coefficient, D,, of PBLG in the hinged rodlike conformation was larger than that for its helical conformation, while D, for the hinged-rodlike PGA was smaller than that for its helical conformation. It is concluded that the hinged-rodlike PGA molecule is in a n extended form and that the hinged-rodlike PBLG is hydrodynamically more compact and rigid than that in its quiescent state. It is deduced that at Co hinged rodlike PBLG molecules collapse t o a conformation optically anisotropic and mechanically rigid.


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Elongational flow-induced birefringence of a T4-phage DNA aqueous solution was measured with changing NaCl and polyethylene glycol (PEG) concentrations. DNA molecules are known to manifest a coil-globule transition with increasing PEG concentration. At certain PEG concentrations near the critical co