Ellipsometric immunosensors for the determination of γ-interferon and human serum albumin

✍ Scribed by T.A. Ruzgas; V.J. Razumas; J.J. Kulys

• Book ID: 103975784
• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 334 KB
• Volume: 7
• Category: Article
• ISSN: 0956-5663
• DOI: 10.1016/0956-5663(92)87009-e

✦ Synopsis

Ellipsometric immunosensors for gamma-interferon (gamma-INF) and human serum albumin (HSA) have been developed using gamma-INF and HSA covalently immobilized onto silicon plates. Detection is achieved by measuring the adsorption decrease (gamma, mg m-2) of monoclonal immunoglobulin G against gamma-INF or immunoglobulin fraction of rabbit blood serum against HSA when the concentration of antigen in mixtures of antibody-antigen is increased. The time of analysis is 50 min, the detection limit of gamma-INF is 15 nM (sensitivity -0.01 mg m-2 nM-1) and of HSA 2.5 nM (-0.06 mg m-2 nM-1).