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ELISA for the detection of serum and saliva IgA against the BMRFI gene product of Epstein-Barr virus

✍ Scribed by Nadala, E. C. B.; Tan, T. M. C.; Wong, H. M.; Ting, R. C. Y.

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
347 KB
Volume
50
Category
Article
ISSN
0146-6615

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✦ Synopsis

The BMRFl protein is an Epstein-Barrvirus (EBV) DNA polymerase accessory protein that forms part of the early antigen diffuse (EA-D) component. An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of IgA antibody to the BMRFI protein of EBV in saliva and serum samples. The assay was shown to be specific for nasopharyngeal carcinoma (NPC) patients and, when used with saliva alone, t o have a sensitivity comparable to an existing indirect immunoperoxidase assay for early antigens. The sensitivity of the assay could be significantly enhanced to 86% by the use of paired saliva and serum samples.

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