Elimination of spin diffusion effects in saturation transfer experiments: application to hydrogen exchange in proteins

✍ Scribed by Malene Ringkjøbing Jensen; Søren M. Kristensen; Jens J. Led

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 166 KB
• Volume: 45
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/mrc.1955

✦ Synopsis

Abstract

The NMR saturation transfer experiment is widely used to characterize exchange processes in proteins that take place on the ms‐s timescale. However, spin diffusion effects are inherently associated with the saturation transfer experiment and may overshadow the effect of the exchange processes of interest. As shown here, the effects from spin diffusion and exchange processes can be separated by varying the field strength of the saturation pulse, thereby allowing correct exchange rates to be obtained. The method is demonstrated using the hydrogen exchange process in the protein Escherichia coli thioredoxin as an example. Copyright © 2007 John Wiley & Sons, Ltd.