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Elimination of Primer–Dimer Artifacts and Genomic Coamplification Using a Two-Step SYBR Green I Real-Time RT-PCR

✍ Scribed by Jo Vandesompele; Anne De Paepe; Frank Speleman


Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
110 KB
Volume
303
Category
Article
ISSN
0003-2697

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✦ Synopsis


The lectin from elderberry bark, Sambucus nigra agglutinin (SNA), 2 and the two lectins from the legume 1 This work was supported by USPHS Grants P01-HL57345 and R01-GM323373.

2 Abbreviations used: SNA, Sambucus nigra agglutinin; MAL, Maackia amurensis leukoagglutinin; MAH, Maackia amurensis FIG. 3. Relative HMBS gene expression levels in nine normal leukocyte samples of which RNA was subjected to two parallel rounds (black and gray) of DNase treatment and cDNA synthesis.