The motor nerve supplying the medial gastrocnemius (MG) muscle was transected in the popliteal fossa of adult cats. The proximal nerve stump was ligated to prevent reinnervation. Three, six or twelve weeks later, axotomized MG motoneurons were intracellularly labelled with horseradish peroxidase, and the morphology of their intramedullary axon collateral systems was investigated quantitatively. The results were compared with corresponding data obtained from normal MG motoneurons. The peripheral chronic axotomy induced a gradual decrease in the number of recurrent axon collaterals originating from the lesioned MG motoraxons within the spinal cord. At 12 weeks postoperatively, this decrease amounted to 40%. The elimination preferentially involved axon collaterals originating from juxta-somatic regions of the motoraxons. In the axon collateral trees persisting in the axotomized MG neurons the tree size, branching patterns and number of synaptic boutons were all normal. Thus, no signs of a gradual deterioration of individual axon collateral systems were observed at any postoperative stage studied. The results are discussed in relation to other retrograde degenerative and regenerative events induced by axotomy.