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Elimination of in vivo cleavage between target protein and intein in the intein-mediated protein purification systems

✍ Scribed by Chunxian Cui; Wentao Zhao; Jianglei Chen; Jianjun Wang; Qianqian Li

Book ID: 116828416
Publisher: Elsevier Science
Year: 2006
Tongue: English
Weight: 391 KB
Volume: 50
Category: Article
ISSN: 1046-5928
DOI: 10.1016/j.pep.2006.05.019

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## Abstract While traditional metabolic engineering generally relies on the augmentation of specific genes and pathways in order to increase the yield of target proteins, the advent of RNA interference (RNAi) as a biological tool has given metabolic engineers another tool capable of rationally alte