Elimination of ColE1 (pBR322 and pBR329) plasmids in Escherichia coli by α-santonin
✍ Scribed by Bharathi, A
- Book ID
- 122249915
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 166 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0378-1097
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The maintenance and genetic stability of the vector plasmids pBR322 and pBR325 in two genetically different Escherichia coli hosts were studied during chemostat cultivation with glucose and ammonium chloride limitation and at two different dilution rates. The plasmid pBR322 was stably maintained und
## Abstract Fermenter studies under batch and fed‐batch conditions were carried out to test the possibility of plasmid pBR322 production in large amounts by using __E. coli relA__ strains. High amplification rates of pBR322 plasmid DNA were observed in __E. coli__ CP79 (__relA__) and __E. coli__ CP