Elimination of by-products in 11β-hydroxylation of Substance S usingCurvularia lunataclones regenerated from NTG-treated protoplasts
✍ Scribed by Danuta Wilmańska; Krystyna Milczarek; Anna Rumijowska; Katarzyna Bartnicka; Leon Sedlaczek
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 500 KB
- Volume
- 37
- Category
- Article
- ISSN
- 1432-0614
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✦ Synopsis
Stable mutants showing improved 11-hydroxylation of Substance S were isolated, following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and regeneration of uninucleate protoplasts of the appropriate fungal strains. This procedure was especially suitable for obtaining more directed 11 beta-hydroxylation of Substance S with Curvularia lunata IM 2901. Apart from producing cortisol (11 beta-hydroxy-S), the parent strain formed several by-products that significantly lowered the yield of the desired 11 beta-hydroxyderivative. Isolated mutants of this microorganism carried out directed 11 beta-hydroxylation with only a small amount of one of the by-products, which resulted in a much higher yield of cortisol.