Elicitation of primary anti-Sendai virus cytotoxic T lymphocytes with purified viral glycoproteins

✍ Scribed by Maria McGee; Arthur H. Hale; Marguerite Panetti

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 575 KB
• Volume: 10
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830101207

✦ Synopsis

Abstract

The requirement of integration of viral proteins into cell surface membranes of host cells for elicitation of anti‐Sendai virus (SV) cytotoxic T lymphocytes (CTL) has been investigated. The purified hemagglutinin‐neuraminidase (HN) and fusion (F) glycoproteins of SV incorporated into phospholipid vesicles was used to analyze this question. Phospholipid vesicles possessing an active HN and F glycoprotein were capable of eliciting both anti‐SV CTL and antibodies. However, the incorporation of an inactive F glycoprotein into HN‐containing vesicles or its absence from such vesicles resulted in stimulation of only anti‐SV antibodies and not anti‐SV CTL.