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Elevated Expression of Free Deaminoneuraminic Acid in Mammalian Cells Cultured in Mannose-Rich Media

✍ Scribed by Takashi Angata; Daisuke Nakata; Tsukasa Matsuda; Ken Kitajima

Book ID
115584136
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
81 KB
Volume
261
Category
Article
ISSN
0006-291X

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## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p