Elevated expression of cd80 (b7/bb1) and other accessory molecules on synovial fluid mononuclear cell subsets in rheumatoid arthritis
✍ Scribed by Erik A. Ranheim; Thomas J. Kipps
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 981 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
Objective. To assess the expression of important accessory molecules such as CDSO (B7/BB1) and CD54 (intercellular adhesion molecule 1) on potential antigenpresenting cells (APC) in the synovial fluid (SF) of patients with rheumatoid arthritis (RA).
Methods. The level of expression of various accessory molecules on T cells, monocytes, and CD5+ or CD5-B cells from RA SF was compared, by multiparameter flow cytometric analysis, with the expression on peripheral blood (PB) mononuclear cells from the same patients and from normal controls. The functional significance of increased expression of these accessory molecules was assessed by the ability of the different cell populations to stimulate T cells in the mixed lymphocyte reaction.
Results. Monocytes, T cells, and B cells isolated from the SF of inflamed joints of patients with RA expressed significantly higher levels of CDSO and CD54 than those from the PB of RA patients or normal controls. CDllb and CDllc expression also were increased on SF B cells, while SF monocytes exhibited enhanced levels of CD40. Further, we found that the elevated CD80 and CD54 levels in RA SF cells may enhance the capacity of these cells to act as stirnulatory APC.
Conclusion. This is the first demonstration that specific cell subsets constitutively express increased levels of CD80 at a site of autoimmune pathology, which Dr.