Elevated expression of calcium-binding protein p9Ka is associated with increasing malignant characteristics of rat prostate carcinoma cells
✍ Scribed by Youqiang Ke; Chun Jing; Roger Barraclough; Paul Smith; Michael P.A. Davies; Christopher S. Foster
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- French
- Weight
- 96 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Northern and Western blotting techniques were used to study expression of the mRNA and corresponding protein product of the S100-related calcium-binding molecule p9Ka in 6 different metastatic cell lines of the Dunning R3327 rat prostate cancer model. In cells with the lowest metastatic capability (G cells), p9Ka mRNA was barely detectable. In 2 weakly metastatic cell lines (AT-1 and AT-2), p9Ka transcript amounts were, respectively, 6.29 6 0.74 and 5.55 6 1.11 times that detected in the G cells. In 3 highly metastatic cell lines (AT-3, MAT-LyLu and MAT-Lu), the amounts of p9Ka mRNA were, respectively, 12.85 6 2.82, 13.06 6 1.69 and 11.62 6 1.81 times that expressed in the G cells. Western blot analyses detected no p9Ka protein in the G cells. The amounts of p9Ka protein expressed by tumour cells of intermediate metastatic capability (AT-1 and AT-2) were 3.4 6 1.3 mg and 3.3 6 1.4 mg, respectively, per 1 3 10 6 cells. The amounts of p9Ka protein expressed by the tumour cells of highest metastatic capability (AT-3, MAT-LyLu and MAT-Lu) were 8.3 6 1.1 mg, 8.7 6 1.6 mg and 9.6 6 1.7 mg, respectively, per 1 3 10 6 cells. Our data reveal a direct association between the elevated expression of mRNA and the p9Ka protein amounts and the increased metastatic capability of individual prostatic cancer cell lines. We suggest that calcium-binding protein p9Ka may play an important role in the metastatic behaviour of rat prostate cancer.