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Elevated activities of protein kinase C and tyrosine kinase correlate to leukemic cell aggressiveness

✍ Scribed by Esther Aflalo; Marina Wolfson; Rivka Ofir; Yacob Weinstein

Publisher
John Wiley and Sons
Year
1992
Tongue
French
Weight
891 KB
Volume
50
Category
Article
ISSN
0020-7136

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✦ Synopsis

We report a linkage between cell aggressiveness, protein kinase C (PKC) activity, tyrosine kinase (PTK) activity and serum requirement. We used 2 leukemic cell lines induced by Moloney murine leukemia virus (MLV). One line was highly aggressive (8s-24-I) and required low serum concentrations (3%) for optimal growth in comparison to the less aggressive line (R02T) that needed 10% serum for optimal growth. The more malignant cells exhibited higher PKC and PTK activity. This activity was independent of serum concentration between 0.01-10%. In contrast, the weakly malignant cells need a high serum concentration (10%) for optimal PKC or PTK activity. lmmunoblot analysis revealed a higher level of PKC protein in the BS-24-1 cells than in the RO2T cells. Serum induction of PKC activity did not change the amount of PKC protein in the cytosol or the membrane fractions, indicating post-translational mechanism regulation of PKC. We suggest that the aggressiveness of BS-24-I resulted from its ability to become independent of growth regulation by serum factors, via autocrine stimulation of PKC and PTK.

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