Elements between the protein-coding regions of the adjacent ?4 and ?3 acetylcholine receptor genes direct neuron-specific expression in the central nervous system
✍ Scribed by Yang, Xiangdong ;Yang, Fei ;Fyodorov, Dmitry ;Wang, Feng ;McDonough, Jennifer ;Herrup, Karl ;Deneris, Evan
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 511 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
The expression patterns of three regions, we investigated the activity of 02732 //47 in clustered neuronal nicotinic acetylcholine receptor vivo. Transgenic mice were generated, which carry the (nAchR) subunit genes ordered b4, a3, and a5 over-lacZ gene fused downstream of 02732 //47. Expreslap extensively in the peripheral nervous system sion of the lacZ transgene is restricted to neurons of (PNS) but only partially in the central nervous system the CNS; no expression was detected in the PNS or (CNS). We have begun to investigate cell type-spein nonneural tissues. LacZ -positive cells were detected cific cis elements regulating these genes by analyzing virtually exclusively in a subset of CNS nuclei that in both cell culture and transgenic mice, a 2.8-kb fragtranscribe the endogenous a3 gene. Some overlap was ment (02732//47) containing the a3 promoter reseen with the b4 gene, but nearly none with the a5 gion, the b4/a3 intergenic region, and a portion of the gene. Our results demonstrate that cis elements posi-b4 3-untranslated exon. The 02732 //47 fragment is tioned between the a3 and b4 coding regions are impreferentially active in PC12 cells relative to nonneuportant for establishing part of the restricted CNS ral cell lines. Deletion analysis revealed a cell typepatterns of b4, a3, and a5 gene transcription. ᭧ 1997 specific positive transcriptional element positioned in