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Electrotransformation of intact and osmotically sensitive cells ofCorynebacterium glutamicum

✍ Scribed by Hendrik Wolf; Alfred Pühler; Eberhard Neumann


Publisher
Springer
Year
1989
Tongue
English
Weight
672 KB
Volume
30
Category
Article
ISSN
1432-0614

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## Abstract Intact human erythrocytes, initially depleted of Mg^2+^ by EDTA incubation in the presence of A23187, exhibit Mg^2+^‐dependent phosphate production of around 1.5 mmol per liter cells · h, half‐maximally activated at around 0.4 mM added free Mg^2+^. This appears to correspond to Mg^2+^‐s