Electrostatic polarization is critical for the strong binding in streptavidin-biotin system

The free energies for mutating S27 and W

The free energies for mutating S27 and W79 to alanine in streptavidin and its biotin complex: The relative size of polar and nonpolar free energies on biotin binding

✍ Richard W. Dixon; Peter Kollman 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 30 KB 👁 3 views

We have carried out calculations on the relative free energy of binding of biotin and its S27A and W79A mutants to streptavidin. Consistent with earlier suggestions by Miyamoto and Kollman from free energy component analysis and recent experiments by Stayton and coworkers, the reduction in binding s