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Electrostatic Contributions to Protein Retention in Ion-Exchange Chromatography. 1. Cytochrome c Variants

✍ Scribed by Yao, Yan; Lenhoff, Abraham M.


Book ID
121076767
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
303 KB
Volume
76
Category
Article
ISSN
0003-2700

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## Abstract Protein elution curves in ion exchange chromatography (IEC) were simulated with a rate model. Three pure proteins and their mixture were used (α‐lactalbumin, BSA, and conalbumin) under different operational conditions. The anionic matrix Q‐Sepharose FF was used packed in a 1 mL column.