Electrospray Mass Spectrometric Characterization of Six Therapeutic Oximes: HI-6, HS-6, Obidoxime, 2-PAM, TMB-4 and HLö-7
✍ Scribed by Paul A. D'Agostino; Lionel R. Provost; James R. Hancock; Camille A. Boulet
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 561 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0951-4198
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✦ Synopsis
Pyridinium and bis-pyridinium oxime salts are currently in use or under development for treatment of patients exposed to organophosphorus nerve agents. The low volatility and thermal lability of these compounds limits the number of mass spectrometric approaches that may be used to characterize them. Electrospray mass spectrometry (ESI-MS), a relatively new ionization approach, was investigated as a possible technique for the characterization and identification of these oximes and their degradation products. Six therapeutic oxime salts, the bis-pyridinium oximes, HI-6, HS-6, obidoxime, TMB-4 and HLO-7, and the pyridinium oxime, 2-PAM, were analysed by ESI-MS, making use of collisionally-activated dissociation (CAD) in the ESI interface. The CAD mass spectrum of each oxime was acquired with two different sampling cone voltage settings and the quadrupole mass analyser associated with a hybrid tandem mass spectrometer was utilized for tandem mass spectrometry (MS/MS) and CAD-MUMS applications including the reliable differentiation of similar bis-pyridinium oximes and the identification of a HI-6 decomposition product.