Electrospray mass spectrometric analysis of poly(ethylene glycol)–protein conjugates
✍ Scribed by Anna Maria Gioacchini; Giacomo Carrea; Francesco Secundo; Mario Baraldini; Aldo Roda
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 97 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0951-4198
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✦ Synopsis
Proteins immobilized on polymer supports are extensively used to develop bioanalytical methods and the determination of the protein/polymer molar ratio is important both for quantifying the catalytic activity and calculating the diffusion rate of the substrates. In this paper a method, based on electrospray mass spectrometry, for characterizing subtilisin and lipase immobilized on chemically modified poly(ethylene glycol)-5000 is described. The results obtained show that this technique is particularly effective. The information provided by it can be used to optimize the synthesis of these polymer-protein conjugates as well as to evaluate the efficiency of their separation and purification.