Electrospray Ionization Mass Spectrometry of Highly Heterogeneous Protein Systems: Protein Ion Charge State Assignment via Incomplete Charge Reduction
β Scribed by Abzalimov, Rinat R.; Kaltashov, Igor A.
- Book ID
- 125980308
- Publisher
- American Chemical Society
- Year
- 2010
- Tongue
- English
- Weight
- 425 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0003-2700
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A method has been developed that takes advantage of the formation of noncovalent compounds in electrospray mass spectrometry. Mixtures of proteins and peptides are shown to produce an intense ion that corresponds to a 1:1 complex with a crown ether (18-crown-6). Although the crown ether may be added
## Abstract The influence of tertiary structure on the electrospray ionization mass spectra of proteins is a well known and broadly exploited phenomenon. However, the underlying mechanism is not well understood. This paper discusses the bases and the implications of the two current hypotheses (solv