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Electroporation of cosmid DNA into bacterial cells

✍ Scribed by Pavel Kubicka; Gordana Kramaric


Book ID
113289841
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
136 KB
Volume
10
Category
Article
ISSN
0168-9525

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Electroporation, the reversible breakdown of cell membranes caused by a high-voltage discharge, is a rapid, simple, and efficient method for introducing DNA into mammalian cells. An instrument for electroporation which permits the high-voltage discharge waveform to be varied with respect to rise tim