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Electroporation-mediated transfection of mammalian cells with crude plasmid DNA preparations

✍ Scribed by Masaaki Tatsuka; Nobuyuki Yamagishi; Morimasa Wada; Hiromi Mitsui; Takahide Ota; Shizuo O-ima

Book ID
113409651
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
455 KB
Volume
12
Category
Article
ISSN
1050-3862

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## Abstract ## Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed transgene expression of transfected supercoiled pDNA concatemers. ## Methods Jurkat T cells were transfected with a supercoi

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We read with interest the article by Maucksch et al. [1], who analysed transgene expression of transfected supercoiled 4.7 kb monomeric and 9.4 kb dimeric plasmid concatemer in Jurkat T cells using electroporation. The authors reported that the relative number of electrotransferred gene copies per n