𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Electroporation: Mechanism and transient expression, stable transformation and biological effects in plant protoplasts

✍ Scribed by Morten Joersbo; Janne Brunstedt


Book ID
110900306
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
748 KB
Volume
81
Category
Article
ISSN
0031-9317

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Factors affecting transient gene express
✍ T. E. Tautorus; F. Bekkaoui; M. Pilon; R. S. S. Datla; W. L. Crosby; L. C. Fowke πŸ“‚ Article πŸ“… 1989 πŸ› Springer 🌐 English βš– 747 KB

Methods were developed for transient gene expression in protoplasts of black spruce (Picea mariana) and jack pine (Pinus banksiana). Protoplasts were isolated from embryogenic suspension cultures of black spruce and from non-embryogenic suspensions of jack pine. Using electroporation, transient expr

Influence of bacterial strain genotype o
✍ Jorge Tovar Torres; Annette Block; Klaus Hahlbrock; Imre E. Somssich πŸ“‚ Article πŸ“… 1993 πŸ› John Wiley and Sons 🌐 English βš– 868 KB

It is demonstrated that transient expression of plasmid DNA in plant protoplasts can be strongly influenced by the bacterial strain used for plasmid propagation. Four different promoter constructs containing two light‐responsive and two fungal elicitor‐responsive parsley promoters translationally fu

Transient gene expression in strawberry
✍ Marie Nyman; Anita Wallin πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 413 KB

A transient ß-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addit