✦ LIBER ✦

Electroporation and PEG delivery of DNA into maize microspores

✍ Scribed by Anne Fennell; Randal Hauptmann

Book ID: 104675351
Publisher: Springer
Year: 1992
Tongue: English
Weight: 392 KB
Volume: 11
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00233094

No coin nor oath required. For personal study only.

✦ Synopsis

The ability to deliver and detect reporter gene activity in maize microspores was tested. Tested expression vectors contained the chloramphenicol acetyl transferase (CAT) gene and one of the following promoter-intron combinations: 1) cauliflower mosaic virus (CaMV 35S), 2) CaMV 35S + maize alcohol dehydrogenase 1 intron 6 (Adh1-I6), 3) maize alcohol dehydrogenase 1 + intron 1 (Adh1-I1), or 4) maize ubiquitin 1 + intron 1 (Ubiq 1-I1) promoter + intron. The expression vectors were delivered into maize microspores using electroporation or polyethylene glycol (PEG). Both methods were effective for delivering free DNA into microspores. Although all four promoters were active in maize protoplasts, only two promoters were active in maize microspores. The CaMV 35S and the Adh1 promoters did not promote gene expression in maize microspore. The CaMV 35S + Adh1-I6 and Ubiq1-I1 promoters produced high levels of CAT activity in maize microspores.

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