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Electropolymerized 1,3-diaminobenzene for the construction of a 1,1′-dimethylferrocene mediated glucose biosensor

✍ Scribed by Robert J. Geise; Sheila Y. Rao; Alexander M. Yacynych

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 620 KB
Volume: 281
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(93)85004-4

No coin nor oath required. For personal study only.

✦ Synopsis

An electron-mediated glucose biosensor was constructed from spectroscopic-grade graphite electrodes in three steps. A nonconducting electropolymerized film serves to screen out electroactive interferents and prevents electrode fouling. The electron mediator l,l'-dimethylferrocene is adsorbed on, and retained by the polymer film-modified electrode. Glucose oxidase is then crosslinked with glutaraldehyde on the modified electrode. This yields a biosensor with linear glucose response (2-76 mM) at + 150 mV vs. SCE, and little or no interference from oxygen, with a lifetime of up to four months.

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