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Electrophoretic transfer of viral proteins to nitrocellulose sheets and detection with peroxidase-bound lectins and protein A

✍ Scribed by Pilar Ramírez; JoséA. Bonilla; Edgardo Moreno; Pedro León


Book ID
118898927
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
442 KB
Volume
62
Category
Article
ISSN
0022-1759

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Glycoprotein detection in nitrocellulose
✍ J.C.S. Clegg 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 810 KB

Glycoproteins in nitrocellulose transfers of electrophoretically separated mixtures of cellular and viral proteins are rapidly and sensitively located by sequential incubation with the lectin concanavalin A and the enzymatically active glycoprotein horseradish peroxidase. The bound enzyme is located