Electrophoretic separation of environmentally important phenolic compounds using montomorillonite-coated fused-silica capillaries

✍ Scribed by Maria F. Mora; Carlos D. García

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 377 KB
• Volume: 28
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200600493

✦ Synopsis

Abstract

This paper reports a simple procedure for coating fused‐silica capillaries with poly(diallyldimethyl ammonium chloride) and montmorillonite. The coated capillaries were characterized by performing EOF measurements as a function of buffer pH, number of layers of coating, and number of runs (stability). The coated capillaries showed a highly stable μ~EOF~ (run‐to‐run RSD less than 1.5%, n = 20), allowing continuous use for several days without conditioning. The coated capillaries were then used for the effective separation of nine environmentally important phenolic compounds showing a significant improvement in the resolution, when compared to bare fused‐silica capillaries. The EOF of the coated capillaries was constant in alkaline solutions (pH ≥ 7), allowing the optimization of the separation conditions of phenolic compounds without significantly affecting the μ~EOF~.