Electrophoretic protein typing ofCampylobacter jejunisubspecies ≪Doylei≫ (nitrate-negative campylobacter-like organisms) from human faeces and gastric mucosa
✍ Scribed by R. J. Owen; M. Costas; L. L. Sloss
- Book ID
- 104642536
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 1013 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0393-2990
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✦ Synopsis
Twenty-seven strains comprising 23 clinical isolates of nitrate negative campylobacters (NNC) from Australia, South Africa, the United Kingdom and the Federal Republic of Germany, a representative of the CNW (catalase negative/weak) group and reference strains of three other Campylobacter species, were characterized by one-dimensional SI)S-polyacrylamide gel electrophoresis of cellular proteins. The protein ,patterns were highly reproducible, and were used as the basis for a numerical analysis which showed that the reference strain (NCTC 11951) o,f Campylobacter jejuni subspecies ~, doylei ,,, ~nd 20 NNC isolates formed a distinct group ,at the 74% similarity level. The protein patterns sho~ved unexpectedly low similarity between subspecies e doyIei ,, and 'the ~ype strain of Campylobacter ]ejuni and revealed that some NNC strains were quite distinct from subspecies , doylei,. Four electrophoretic (EP) types (I-IV) were identified from phenons formed at the 81% similarity level. Three of these (I, III, IV) corresponded to geographical location of strain isolation but the type II strains were from diverse locations. The correlation observed between EP-type, catalase ,production and sensitivity to 2, 3, 5, ~riphenyltetrazolium chloride indicated these latter two tests might be useful for biotyping within the subspecies.