Electrophoretic method for separating small peptides in serum without extraction of macromolecules: Application to the detection of preeclampsia

A rapid and simple electrophoretic metho

A rapid and simple electrophoretic method for the detection of mutations involving small insertion or deletion: application to β-thalassemia

✍ Shi-Ping Cai; Barry Eng; Yuet Wai Kan; David H. K. Chui 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 581 KB

The 1.8-kb beta-globin gene fragments of DNAs from individuals heterozygous for nine different beta-thalassemia mutations involving 1, 2, 3, 4, or 25 basepair (bp) insertions or deletions were amplified by the polymerase chain reaction (PCR). The PCR products were subjected to electrophoresis on aqu