Polarized dynamic light-scattering measurements on superhelical pBR322-plasmid DNA solutions in 0.2M NaC1,2 mM NaPi, pH 7.0,2 mM EDTA result in a translational diffusion coefficient D$, , . c = (3.77 f 0.10) X cmz/s for the native molecule. Modeling the DNA, in the simplest approximation, as a 10 X
Electrophoretic light scattering as a probe of reaction kinetics
β Scribed by B. J. Berne; Rina Giniger
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1973
- Tongue
- English
- Weight
- 346 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0006-3525
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β¦ Synopsis
Abstract
A new method is proposed for determining chemical rate contants in dimerization reactions of globular proteins. Light scattering from a solution of charged macromolecules in and applied electric field gives a series of bands whose widths can be used to deduce the reaction rate contants. This method should be applicable to other types of peactions. First order reactions are also considered.
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