Electrophoretic identification of hard white spring wheats grown at different locations in Pakistan in different years

✍ Scribed by Faqir M Anjum; G L Lookhart; C E Walker

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 247 KB
• Volume: 80
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/1097-0010(200006)80:8<1155::aid-jsfa587>3.0.co;2-j

✦ Synopsis

Pakistani wheats were identi®ed via acid polyacrylamide gel electrophoresis (A-PAGE) of gliadin proteins. Gliadin electrophoretograms of wheat cultivars were qualitatively independent of growth locations and years. The Pakistani wheat Faisalabad 83 and the American wheat KS84HW196 contained an intense gliadin band in the region of the relative mobility 50 band of Marquis. That band was readily distinguishable and was followed by distinct intense double bands in the same position as those of Marquis, and can be used as a reference for mobility assignments in wheat cultivar identi®cation work. Minor differences in band intensities across locations and years were noticed. Identi®cation tables based on band mobilities relative to Marquis and relative intensities were established.