Electrophoresis of uronic acids, neutral sugars and hydrolysates of acidic polysaccharides on silylated glass-fibre paper in electrolytes of bivalent cations

✍ Scribed by Beat Bettler; Renato Amadò; Hans Neukom

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 1001 KB
• Volume: 498
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84250-3

✦ Synopsis

The separation of different uranic acids, oligogalacturonic acids and neutral sugars by electrophoresis on silylated glass-fibre paper is described. As electrolytes 0.1 M solutions of barium, zinc and calcium acetate were used. In these solutions the separation of acid constituents was possible without interference from neutral sugars. Depending on the electrolytes, a mixture of galacturonic, mannuronic, glucuronic and guluronic acid was separatad within 90-165 min. The partial characterization of acid polysaccharides such as pectin, alginate and mixtures thereof is described.