𝔖 Bobbio Scriptorium
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Electrophoresis of ribonuclease: isoelectric points on various adsorbents.

Publisher
Elsevier
Year
1960
Weight
64 KB
Volume
1
Category
Article
ISSN
0368-1874

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Determination of isoelectric point value
Determination of isoelectric point value of 3-mercaptopyruvate sulfurtransferase by isoelectric focusing using ribonuclease A-glutathione mixed disulfides as standards
✍ Toshihiko Ubuka; Noriyoshi Masuoka; Shigeko Yoshida; Kazushi Ishino πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 626 KB

The pI value of rat erythrocyte 3-mercaptopyruvate sulfm-transferase (EC 2.8.1.2) was determined to be 5.9 at 10Β°C by isoelectric focusing in a horizontal slab polyacrylamide gel containing 2% carrier ampholyte (pH 3-10). In this study, ribonuclease A-glutathione mixed disulfides (RNase-SG's) (T. Ub